| Animal
Biosafety Level 1 (back
to Safety Table)
A.
Standard Practices
1.
Access to the animal facility is limited or restricted at the
discretion of the laboratory or animal facility director.
2.
Personnel wash their hands after handling cultures and animals,
after removing gloves, and before leaving the animal facility.
3.
Eating, drinking, smoking, handling contact lenses, applying cosmetics,
and storing food for human use are not permitted in animal rooms.
Persons who wear contact lenses in animal rooms should also wear
goggles or a face shield.
4.
All procedures are carefully performed to minimize the creation
of aerosols.
5.
Work surfaces are decontaminated after use or after any spill
of viable materials.
6.
Doors to animal rooms open inward, are self-closing and are kept
closed when experimental animals are present.
7.
All wastes from the animal room are appropriately decontaminated,
preferably by autoclaving, before disposal. Infected animal carcasses
are incinerated after being transported from the animal room in
leakproof, covered containers.
8.
An insect and rodent control program is in effect.
B.
Animal Facilities (Secondary
Barriers)
1.
The animal facility is designed and constructed to facilitate
cleaning and housekeeping.
2.
A handwashing sink is available in the animal facility.
3.
If the animal facility has windows that open, they are fitted
with fly screens.
4.
Exhaust air is discharged to the outside without being recirculated
to other rooms, and it is recommended, but not required, that
the direction of airflow in the animal facility is inward.
C.Special
Practices
1.
The laboratory or animal facility director limits access to the
animal room to personnel who have been advised of the potential
hazard and who need to enter the room for program or service purposes
when work is in progress. In general, persons who may be at increased
risk of acquiring infection, or for whom infection might be unusually
hazardous, are not allowed in the animal room.
2.
The laboratory or animal facility director establishes policies
and procedures whereby only persons who have been advised of the
potential hazard and meet any specific requirements (e.g., immunization)
may enter the animal room.
3.
Bedding materials from animal cages are removed in such a manner
as to minimize the creation of aerosols, and are disposed of in
compliance with applicable institutional or local requirements.
4.
Cages are washed manually or in a cage washer. Temperature of
final rinse water in a mechanical washer should be 180 degrees
F.
5.
The wearing of laboratory coats, gowns, or uniforms in the animal
facility is recommended. It is further recommended that laboratory
coats worn in the animal facility not be worn in other areas.
6.
A biosafety manual is prepared or adopted. Personnel are advised
of special hazards, are required to read and to follow instructions
on practices and procedures.
D.
Safety Equipment (Primary Barriers)
Special
containment equipment is not required for animals infected with
agents assigned to Biosafety Level 1.
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