BSL
LEVEL |
AGENTS
|
PRACTICES
|
SAFETY
EQUIPMENT (Primary Barriers) |
FACILITIES
(Secondary Barriers) |
|
Not known
to cause disease in healthy human adults. |
Standard
animal care and management practices, including appropriate
medical surveillance programs. |
As required
for normal care of each species. |
Standard
animal facility:
*non
recirculation of exhaust air;
*directional air flow recommended.
|
|
Associated
with human disease. Hazard: percutaneous exposure, ingestion,
mucous membrane exposure. |
BSL-1
practices plus:
*limited access;
*biohazard warning signs;
*sharps precautions;
*biosafety manual;
*decontamination of all infectious wastes and of animal cages
prior to washing. |
BSL-1
equipment plus primary barriers: containment equipment
appropriate for animal species; PPEs: laboratory coats, gloves,
face and respiratory protection as needed. |
BSL-1
facility plus:
*autoclave available;
*handwashing sink available in the animal room. |
|
Indigenous
or exotic agents with potential for aerosol transmission; disease
may have serious health effects. |
BSL-2
practices plus:
*controlled access;
*decontamination of clothing before laundering;
*cages decontaminated before bedding removed;
*disinfectant foot bath as needed. |
ABSL-2,
equipment plus:
*containment equipment for housing animals and cage dumping
activities;
*Class I or II BSCs available for manipulative procedures (inoculation,
necropsy) that may create infectious aerosols. PPEs: appropriate
respiratory protection. |
BSL-2,
facility plus:
*physical separation from access corridors;
*self-closing, double-door access;
*sealed penetrations;
*sealed windows
*autoclave available in facility. |
|
Dangerous/exotic
agents which pose high risk of life threatening disease; aerosol
transmission, or related agents with unknown risk of transmission.
|
BSL-3
practices plus:
*entrance through change room where personal clothing is removed
and laboratory clothing is put on; shower on exiting;
*all wastes are decontaminated before removal from the facility.
|
BSL-3
equipment plus:
*Maximum containment equipment (i.e., Class III BSC or partial
containment equipment in combination with full body, air-supplied
positive-pressure personnel suit) used for all procedures and
activities. |
BSL-3
facility plus:
*separate building or isolated zone;
*dedicated supply/exhaust, vacuum and decontamination systems.
*other requirements outlined in the text |
BSL
LEVEL |
AGENTS
|
PRACTICES
|
SAFETY
EQUIPMENT (Primary Barriers) |
FACILITIES
(Secondary Barriers) |
