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Buprenorphine Usage

Buprenorphine: Trade name: Buprenex - Strength: 0.3 mg/ml in 1 ml ampules

Buprenorphine is a narcotic agonist/antagonist with activity primarily at the u opiate receptor. This drug may cause mild anorexia and slight hyperactivity in rodents.


Rat dose: 0.01 - 0.05 mg/kg

1) Dilute Buprenex 1:5 in sterile saline or water (1cc buprenex solution plus 4 cc sterile water or saline).
2) Store diluted Buprenex in sterile vial or sterile vacutainer and label appropriately (name, strength, exp. date, lot number.) NOTE: Federal regulations require that any vial or container used for keeping drugs is labeled with


1) Drug Name,
2) Strength,
3) Manufacturing Lot Number, and
4) the Expiration Date.


3) Store in locked cabinet.
4) Administer at rate of 0.05 ml/ 100 gm BW (i.e., 0.1ml for 200 gm rat) subcutaneously. This provides an analgesic dosage of 0.03 mg/kg BW - an average analgesic dosage appropriate in most post-surgical situations.
5) Best timing of administration is BEFORE incision, i.e., during anesthesia induction.
6) Analgesic activity is present up to 12 hours.
7) Rat should be re-checked for signs of pain or discomfort 12 hours after initial dose to determine need for additional analgesic.

Notes:

  • Buprenorphine is an opioid analgesic with excellent analgesic activity.
  • Respiratory depression is not usually a problem with this opioid administered at analgesic levels.
  • Opioid analgesics may reduce the quantity of primary anesthetic administered.
  • Multiple doses of buprenorphine in rats has occasionally been associated with a tendency of the rats to ingest bedding and subsequently exhibit decreased appetite for food. The reason for this is as yet unexplained.
  • For this reason, no more than two sequential doses is recommended. If the rat has not responded to the analgesic by this time, it is not likely to do so, and euthanasia should be considered.

Mouse dose: 0.05 - 0.2 mg/kg

1) Dilute Buprenex 1:10 in sterile saline or water
2) store diluted Buprenex in sterile vacutainer or 10 cc sterile vial and label appropriately (Name, strength, exp. Date, Drug No.)
3) Store in locked cabinet
4) Administer at rate of 0.1 cc / 30 gm mouse subcutaneously (Delivered dose; 0.1 mg/kg).
5) Best timing of administration is BEFORE incision, i.e., during anesthesia induction.
6) Analgesic activity is present up to 12 hours.
7) Mice should be re-checked for signs of pain or discomfort 12 hours after initial dose to determine need for additional analgesic.

Above information provided by Dan Theele, DVM, PhD, U New Mexico.


  • The dosage for this drug is 0.1 – 0.5 mg/kg subcutaneously every 4 to 6 hours. Due to delayed onset of action, buprenorphine should be administered at least 20 minutes prior to the completion of a surgical procedure.
  • To prepare a dilution for smaller rodents, one 1 ml vial of Buprenex (0.3 mg) is drawn into a sterile syringe and added to 19 ml of sterile 5% dextrose in water or 0.9% NaCl and dispensed into a sterile vial (e.g. a sterile serum tube). Label the tube "Buprenorphine HCl", 0.015 mg/ml and the date prepared. Expiration date is either that from the original package, or 6 months from the date prepared. The solution must be protected from light.
  • Administer 0.06-0.13 ml subcutaneously to an average 20 gram mouse, 0.6-1.3 ml per average 200g rat. There is a wide range of safety and efficacy for this drug. It is not necessary to weigh mice prior to administration and doses may be doubled if clinical efficacy is not apparent.

Above information taken from the Harvard Medical School site.

 
 
                     
                         
                         
 
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